The nutrient medium typically includes macronutrients, micronutrients, vitamins, amino acids, and a carbon source (usually sucrose). The most common is the Murashige and Skoog (MS) medium. D. Growth Regulators
[ Plant Tissue Culture Environment ] │ ┌───────────────┼───────────────┐ ▼ ▼ ▼ [ Nutrient Medium ] [ Sterilization ] [ Microclimate ] - Macro/Micro - Autoclaving - 25°C Temp - Vitamins/Sugar - Chemical Clean - 16hr Light - Hormones (PGRs) - Laminar Flow - 50-60% RH 1. Laboratory Layout and Infrastructure plant tissue culture ppt pdf
Choosing a healthy, disease-free mother plant. The nutrient medium typically includes macronutrients