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Understanding the underlying science helps ensure a successful extraction. 1. Cell Lysis via Freezing
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The non-polar solvent is warmed slightly and added to the vessel. The vessel is gently rolled or inverted end-over-end to ensure the solvent contacts the basic solution without creating a permanent emulsion. The layers are allowed to separate, and this mixing process is repeated 4 to 5 times over several hours. Step 6: Freeze Precipitation cybs max ion tek pdf free full